HPLC Column, Bidentate C8 2.o, 2.2um, 2.1mm ID x 150mm Length, 120A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40208‑15P‑2 Cogent Bidentate C8™ 2.o HPLC Column is a long‑format, narrow‑bore, high‑efficiency reversed‑phase column designed for applications requiring maximum resolution with alternative selectivity. Built on Cogent TYPE‑C™ silica hydride technology, this column combines 2.2 µm particles with bidentate C8 bonding to deliver excellent selectivity, faster equilibration, and near‑UHPLC performance on appropriately configured LC systems.
The Bidentate C8™ 2.o stationary phase is particularly well suited for hydrophobic and non‑polar compounds that are difficult to retain in aqueous mobile phases but may be over‑retained on C18 columns. The shorter alkyl chain moderates hydrophobic interaction strength while preserving the chemical stability and reduced silanol activity inherent to TYPE‑C™ silica hydride surfaces.
The 2.1 mm internal diameter supports low‑flow operation, reduced solvent consumption, and enhanced MS sensitivity. The extended 150 mm length provides the highest resolving power in the analytical C8™ 2.o lineup, making this column a strong choice for LC‑MS workflows requiring separation of closely eluting components. Because of the narrow‑bore format and increased backpressure associated with smaller particles and longer length, use requires an LC system designed for 2.1 mm ID columns.
Why Choose the 2.1 × 150 mm Bidentate C8™ 2.o Format
This column is typically selected when:
C18 columns provide excessive retention or mask selectivity differences
Maximum analytical resolution is needed for hydrophobic or non‑polar analytes
Shorter C8™ 2.o columns (50–100 mm) do not fully resolve critical pairs
LC‑MS methods demand improved separation without migrating to C18 chemistry
The combination of long length and TYPE‑C™ silica hydride chemistry delivers high plate count while maintaining fast equilibration and long column life.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than conventional silica‑based C8 columns
Excellent chemical robustness and reproducibility
Bidentate C8™ 2.o Bonding
Two‑point attachment via direct Si–C bonds
Alternative reversed‑phase selectivity when C18 is too retentive
Controlled hydrophobic retention for difficult analytes
Near‑UHPLC Efficiency (2.2 µm)
Increased efficiency vs. 3–5 µm particle columns
High performance on properly configured HPLC systems
Improved resolution and peak capacity
LC‑MS‑Optimized Geometry
2.1 mm ID for enhanced MS sensitivity and solvent reduction
150 mm length for maximum analytical resolution
Well suited for ESI‑MS and LC‑MS/MS applications
System Requirements
Due to the 2.1 mm ID, small‑particle, long‑format design, optimal performance requires:
An LC pump capable of precise, stable low‑flow delivery
System components (injector, tubing, detector flow cell) designed for 2.1 mm ID columns
Systems not designed for low‑flow analytical operation may not achieve expected performance.
Typical Applications
LC‑MS and LC‑MS/MS
Hydrophobic and non‑polar compounds
Alternative selectivity when C18 is overly retentive
High‑resolution reversed‑phase separations
Advanced method development and optimization